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| Larcher, Thibaut; Perrichon, Prescilla; Vignet, Caroline; Ledevin, Mireille; Le Menach, Karyn; Lyphout, Laura; Landi, Laure; Clerandeau, Christelle; Lebihanic, F.; Menard, Dominique; Burgeot, Thierry; Budzinski, Helene; Akcha, Farida; Cachot, J.; Cousin, Xavier. |
| Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous contaminants that can be present at high levels as mixtures in polluted aquatic environments. Many PAHs are potent mutagens and several are well-known carcinogens. Despite numerous studies on individual compounds, little is known about the toxicity of PAHs mixtures that are encountered in environmental situations. In the present work, zebrafish were continuously fed from 5 days post-fertilisation to 14 months post-fertilisation (mpf) with a diet spiked with fractions of either pyrolytic (PY), petrogenic light oil (LO), or petrogenic heavy oil (HO) origin at three concentrations. A decrease in survival was identified after 3 mpf in fish fed with the highest concentration of HO or LO, but not for PY. All... |
| Tipo: Text |
Palavras-chave: Polycyclic aromatic hydrocarbon; Zebrafish; Carcinogenesis; Genotoxicity; Neoplasia; Carcinoma; Toxicological pathology. |
| Ano: 2014 |
URL: https://archimer.ifremer.fr/doc/00242/35329/34108.pdf |
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| Vignet, Caroline; Le Menach, Karyn; Mazurais, David; Lucas, Julie; Perrichon, Prescilla; Le Bihanic, Florane; Devier, Marie-hélène; Lyphout, Laura; Frere, Laura; Begout, Marie-laure; Zambonino-infante, Jose Luis; Budzinski, Helene; Cousin, Xavier. |
| The release of polycyclic aromatic hydrocarbons (PAHs) into the environment has increased very substantially over the last decades leading to high concentrations in sediments of contaminated areas. To evaluate the consequences of long-term chronic exposure to PAHs, zebrafish were exposed, from their first meal at 5 days post fertilisation until they became reproducing adults, to diets spiked with three PAH fractions at three environmentally relevant concentrations with the medium concentration being in the range of 4.6–6.7 μg g−1 for total quantified PAHs including the 16 US-EPA indicator PAHs and alkylated derivatives. The fractions used were representative of PAHs of pyrolytic (PY) origin or of two different oils of differing compositions, a heavy fuel... |
| Tipo: Text |
Palavras-chave: Danio rerio; Life cycle; Polycyclic aromatic hydrocarbon; Heavy oil; Light crude oil; Alkylated PAH; Jaw; Digestion. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00188/29907/28490.pdf |
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| Le Bihanic, Florane; Perrichon, Prescilla; Landi, Laure; Clerandeau, Christelle; Le Menach, Karyn; Budzinski, Helene; Cousin, Xavier; Cachot, Jerome. |
| Most persistent organic pollutants, due to their hydrophobic properties, accumulate in aquatic sediments and represent a high risk for sediment quality. To assess the toxicity of hydrophobic pollutants, a novel approach was recently proposed as an alternative to replace, refine and reduce animal experimentation: the medaka embryo–larval sediment contact assay (MELAc). This assay is performed with Japanese medaka embryos incubated on a natural sediment spiked with the compound being tested. With the aim of improving this assay, our study developed a reference exposure protocol with an artificial sediment specifically designed to limit natural sediment composition uncertainties and preparation variability. The optimum composition of the new artificial... |
| Tipo: Text |
Palavras-chave: Artificial sediment; Spiked sediment; PAHs; Japanese medaka embryos; Embryotoxicity; Teratogenicity. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00187/29872/28479.pdf |
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| Perrichon, Prescilla; Le Bihanic, Florane; Bustamante, Paco; Le Menach, Karyn; Budzinski, Helene; Cachot, Jerome; Cousin, Xavier. |
| Due to hydrophobic and persistent properties, polycyclic aromatic hydrocarbons (PAHs) have a high capacity to accumulate in sediment. Sediment quality criteria, for the assessment of habitat quality and risk for aquatic life, include understanding the fate and effects of PAHs. In the context of European regulation (REACH and Water Framework Directive), the first objective was to assess the influence of sediment composition on the toxicity of two model PAHs, benzo[a]pyrene and fluoranthene using 10-day zebrafish embryo-larval assay. This procedure was undertaken with an artificial sediment in order to limit natural sediment variability. A suitable sediment composition might be then validated for zebrafish and proposed in a new OECD guideline for chemicals... |
| Tipo: Text |
Palavras-chave: Fish embryo-larval assay; PAHs; Artificial sediment; EROD; Behavioral performance; Teratogenicity. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00242/35323/37905.pdf |
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| Perrichon, Prescilla; Akcha, Farida; Le Menach, Karyn; Goubeau, Manon; Budzinski, Helene; Cousin, Xavier; Bustamante, Paco. |
| In recent decades, PAH emissions due to extensive anthropogenic activities have risen sharply causing considerable pollution of aquatic ecosystems. This pollution represents a threat for organisms, among them are fish. Consequently, prenatal stress can have important repercussions, and may impact survival and population recruitment. To investigate this point, eggs were collected from zebrafish exposed during 6 months by trophic route to three aromatic fractions from two different origins, pyrolytic (PY) and petrogenic (light (BAL) and heavy (HFO) fractions) sources. Chronic dietary exposure of the parents was performed at environmentally relevant concentrations (0.3 ×, 1 × and 3 ×; 1 × represents an environmental concentration measured in French estuary).... |
| Tipo: Text |
Palavras-chave: Polycyclic aromatic hydrocarbons; Parental transmission; Fish early-life stages; PhotoMotor Response; Cardiac performance; PAH metabolites; Genotoxicity. |
| Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00259/37046/37174.pdf |
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| Perrichon, Prescilla; Le Menach, Karyn; Akcha, Farida; Cachot, Jerome; Budzinski, Helene; Bustamante, Paco. |
| Petroleum compounds from chronic discharges and oil spills represent an important source of environmental pollution. To better understand the deleterious effects of these compounds, the toxicity of water-accommodated fractions (WAF) from two different oils (brut Arabian Light and Erika heavy fuel oils) were used in this study. Zebrafish embryos (Danio rerio) were exposed during 96 h at three WAF concentrations (1, 10 and 100% for Arabian Light and 10, 50 and 100% for Erika) in order to cover a wide range of polycyclic aromatic hydrocarbon (PAH) concentrations, representative of the levels found after environmental oil spills. Several endpoints were recorded at different levels of biological organization, including lethal endpoints, morphological... |
| Tipo: Text |
Palavras-chave: Zebrafish embryos; Oil exposure; Teratogenicity; Swimming performance; Bradycardia; Detoxification pathway. |
| Ano: 2016 |
URL: http://archimer.ifremer.fr/doc/00342/45324/46430.pdf |
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